| 摘要 |
<p>Disclosed is an in vitro method for detecting early seroconversion in a body component of a mammalian host to hepatitis C virus, HCV, comprising the steps of: (a) contacting said body component suspected of containing antibodies to HCV with (1) an HCV antigen selected from an antigen encoded in the E1 domain of an HCV genome, the E2 domain of an HCV genome or aggregates thereof, wherein said HCV antigen is purified under non-denaturing conditions or (2) a standard control, under conditions that allow an immunological reaction to occur and (b) detecting the presence of immune complexes of said antibodies and said antigen, wherein the absence of said immune complexes containing the standard control and the presence of said immune complexes containing said HCV antigen indicate seroconversion to HCV. Disclosed is also a kit for use in the method listed above, wherein said kit comprises said HCV antigen and control standards packaged in suitable vials and instruction for use of the kit components.</p> |
