发明名称 |
PLANT NUCLEOTIDE-SUGAR PYROPHOSPHATASE/PHOSPHODIESTERASE (NPPASE), METHOD OF OBTAINING SAME AND USE OF SAME IN THE PRODUCTION OF ASSAY DEVICES AND IN THE PRODUCTION OF TRANSGENIC PLANTS |
摘要 |
NPPase is an enzyme that catalyses the hydrolysis of a wide range of small molecules with phosphodiester and phosphosulphate bonds, in particular ADPG (adenosine diphosphate glucose) and APS (adenosine 5'-phosphosulphate). The enzyme obtained from plant extracts is used in assay devices for determining levels of nucleoside diphosphate sugars, based either on the sugar-1-phosphate released, or on the nucleoside monophosphate, both of which are products formed by the reaction catalysed by NPPase, as well as the detection of sulphonucleotides such as 3'-phosphoadenosine 5'-phosphosulphate (PAPS) and APS. The amino acid sequence of the enzyme is also described, as well as the nucleotide sequence of a complete cDNA and another incomplete cDNA. Finally, it describes the production of transgenic plants that overexpress NPPase and that have a high content of sugars, low content of starch and cell-wall polysaccharides and high resistance to high concentrations of salts and high temperature.
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申请公布号 |
EP1535998(A1) |
申请公布日期 |
2005.06.01 |
申请号 |
EP20030740519 |
申请日期 |
2003.07.15 |
申请人 |
UNIVERSIDAD PUBLICA DE NAVARRA;NIIGATA UNIVERSITY;CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS |
发明人 |
MUNOZ PEREZ, FRANCISCO JOSE;RODRIGUEZ LOPEZ MILAGROS;BAROJA FERNANDEZ, MIREN EDURNE;POZUETA ROMERO, FRANCISCO JAVIER;MITSUI, TOSHIAKI;NANJO, YOHEI |
分类号 |
A01H5/00;B01D57/02;B03C5/00;C12M1/34;C12N9/14;C12N9/16;C12N15/09;C12N15/55;C12Q1/34;C12Q1/42;C12Q1/44;(IPC1-7):C12N9/16;C12N15/82 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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