发明名称 |
HANSENULA POLYMORPHA YAPSIN DEFICIENT MUTANT STRAIN AND PROCESS FOR THE PREPARATION OF RECOMBINANT PROTEINS USING THE SAME |
摘要 |
The present invention relates to a nucleic acid molecule comprising the HpYPS1 gene encoding H. polymorpha yapsin1, a polypeptide encoded by the nucleic acid molecule, a H. polymorpha mutant strain having reduced yapsin activity by mutation of the HpYPS gene encoding H. polymorpha yapsin1, a recombinant H. polymorpha strain expressing a foreign protein produced by introducing a gene encoding the foreign protein into the H. polymorpha mutant strain, and a process for preparing a foreign protein comprising culturing the recombinant H. polymorpha strain under conditions to express the foreign protein and isolating the foreign protein from the culture. |
申请公布号 |
EP1534835(A1) |
申请公布日期 |
2005.06.01 |
申请号 |
EP20030736354 |
申请日期 |
2003.06.28 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;LEADBIO INC.;BIOHOLDINGS CO., LTD. |
发明人 |
KANG, HYUN-AH;RHEE, SANG-KI;SOHN, MIN-JEONG;KIM, JEONG-YOON |
分类号 |
C12N9/58;C12N15/31;C12N15/81;C12P21/02;C12P21/06 |
主分类号 |
C12N9/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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