Method of determining blood-relationship by typing str alleles on the x chromosome and dna typing kit using the same

摘要

The present invention relates to a method for determining blood-relationship by typing STR alleles on the X chromosome and a DNA typing kit using the same, particularly when the father's DNA is not present. According to the method of the present invention, the blood-relationship of a suspected-grandmother and a suspected-granddaughter can be confirmed in the absence of the father's DNA by comparing STR alleles on the X chromosome of each subject. As for step-sisters born of different mothers, their blood-relationship can be determined by investigating whether they share STR alleles on the X chromosome when their mothers' alleles on the X chromosome were excluded. Therefore, the method of the present invention can be usefully used in determining the blood-relationship in the absence of the father's DNA.