摘要 |
Methods for assay and/or amplification of nucleic acid sequences. An amplification procedure is provided for use with RNA samples that do not have a poly A sequence on the 3 prime end of the messenger RNA as is the case for bacterial, and other total RNA sources. Further, a method is provided for coupling, via ligation, a nucleic acid sequence to the 5 prime end of a random or sequence specific primer or to the 3 prime or 5 prime end of a synthesized DNA probe or target sequence, preferably to enable labelling of the target sequence or amplification thereof. |
申请人 |
DATASCOPE INVESTMENT CORP.;GETTS, ROBERT, C.;KADUSHIN, JAMES;SCHWALM, JAIME;HOWERTON, KEVIN |
发明人 |
GETTS, ROBERT, C.;KADUSHIN, JAMES;SCHWALM, JAIME;HOWERTON, KEVIN |