发明名称 |
Method for isolating and purifying nucleic acid, cartridge for isolating and purifying nucleic acid, and kit isolating and purifying nucleic acid |
摘要 |
The invention provides a method for isolating and purifying nucleic acids, which comprises: (1) passing a sample solution containing a nucleic acid through a nucleic acid adsorbing porous membrane to adsorb the nucleic acid to the nucleic acid adsorbing porous membrane; (2) passing a washing solution through the nucleic acid adsorbing porous membrane to wash the nucleic acid adsorbing porous membrane while adsorbing the nucleic acid; and (3) passing an elution solution through the nucleic acid adsorbing porous membrane to desorb the nucleic acid from the nucleic acid adsorbing porous membrane, wherein the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing the nucleic acid by interaction involving substantially no ionic bond, and a step of drying the nucleic acid adsorbing porous membrane adsorbing the nucleic acid is not included between the washing step (2) and the recovering step (3).
|
申请公布号 |
US2005112656(A1) |
申请公布日期 |
2005.05.26 |
申请号 |
US20040974681 |
申请日期 |
2004.10.28 |
申请人 |
FUJI PHOTO FILM CO., LTD. |
发明人 |
IWAKI YOSHIHIDE |
分类号 |
C12N15/09;B01D15/08;B01D15/42;B01D61/00;B01D69/12;B01D71/16;B01J20/02;B01J20/24;B01J20/28;C07H21/00;C07H21/04;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|