| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for three-dimensional culture of hepatocyte and a method for culturing a hepatocyte with which physiologically active substances such as serum albumin,α-fetoprotein, thrombopoietin, cytochrome P450, pregnane X receptor (PXR), carboxylase, hepatocyte nuclei factor 4α(HNF-4α), etc., induced from a hepatocyte are mass produced in vitro three-dimensionally. <P>SOLUTION: The method for culturing a hepatocyte comprises supplying a culture solution to a three-dimensional cell culture apparatus charged with a three-dimensional cell culture carrier, supporting a hepatocyte on the carrier, continuously supplying the culture solution to proliferate the hepatocyte and producing the physiologically active substances from the hepatocyte. In the method for culturing the hepatocyte, the established cell line of the hepatocyte is a FLC series strain. <P>COPYRIGHT: (C)2005,JPO&NCIPI</p> |
