| 摘要 |
PROBLEM TO BE SOLVED: To obtain an improved composition and to provide an improved method for modulating mitochondria calcium homeostasis in consideration of the importance of mitochondria modulation with intracellular calcium and the relationship of mitochondria activity with disease conditions, and to provide an assay for specifically detecting medicines for changing mitochondria calcium modulation so as to provide improved treatment of these diseases. SOLUTION: The method for identifying medicines for changing mitochondria functions comprises following steps; (a) a step for contacting, in each of reaction vessels in a high throughput screening array, (i) a biological sample containing cells comprising cytosol, mitochondria and calcium indicator molecules with (ii) a source of calcium cation under a condition capable of keeping mitochondria membrane potential, wherein the calcium indicator molecules can generate detectable signal proportional to calcium level in the cytosol, (b) a step for detecting, in each reacting vessel and at plural time points, signals generated by the calcium indicator molecules, (c) a step for comparing signals generated by the calcium indicator molecules in the absence of the candidate medicine at one or more time points with signals generated by the calcium indicator molecules in the presence of the candidate medicine at one or more time points and, thereby, identifying the medicine for changing mitochondria functions. COPYRIGHT: (C)2005,JPO&NCIPI
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