| 摘要 |
A DNA amplification method is disclosed, comprising (a) denaturing a DNA to separate a double-stranded DNA into two single-stranded DNA, (b) hybridizing two types of oligonucleotides which were designed so that the end of the 3' side of the region of a sequence to be amplified is identified and hybridized with each single-stranded DNA that has been separated, (c) elongating the oligonucleotides with a DNA polymerase in the presence of deoxyribonycleotide triphosphate, and (d) repeating steps (a) through (c) to perform DNA amplification, wherein in step (b), the two types of oligonucleotides are oligonucleotides in which a polymer having a lower critical solution temperature is linked to the 5' end.
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