发明名称 |
LATE-PCR |
摘要 |
A non-symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays. |
申请公布号 |
EP1468114(A4) |
申请公布日期 |
2005.05.25 |
申请号 |
EP20020795963 |
申请日期 |
2002.12.19 |
申请人 |
BRANDEIS UNIVERSITY |
发明人 |
WANGH, LAWRENCE, J.;PIERCE, KENNETH;HARTSHORN, CRISTINA;RICE, JOHN;SANCHEZ, J., AQUILES |
分类号 |
G01N33/53;C07H21/00;C12N15/09;C12Q1/68;G01N1/28;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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