Method for measuring fluorescence, apparatus for measuring fluorescence and apparatus for evaluating sample using it

摘要

In a fluorescence measuring method and apparatus, a sample S is irradiated with pulsed pumping light supplied from a pumping light source. Fluorescence generated by the sample S is detected by a photodetector by way of a condensing optical system and a spectroscope. The fluorescence time waveform is subjected to a data analysis in a data processing unit in a controller. This computes waveform data and physical quantities such as fluorescence lifetime. The pumping light time waveform is fixedly arranged with respect to a time axis used for data analysis. Fitting calculations are carried out while moving the fluorescence time waveform and fitting range from an initial position earlier than a pumping light peak to a later end position, and optimal measurement waveform data is selected according to a predetermined selection criterion. Waveform data is thus computed accurately and efficiently regardless of fluctuations in the fluorescence time waveform.