Method of screening oxysterol activation of LXRalpha

摘要

The present invention provides a method of screening for agonists of an oxycholesterol activator of LXRalpha transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; treating the host cell with potential LXR-specific ligands; and identifying compounds which activate LXRalpha transcription. Also provided is a method of screening for antagonists of an oxycholesterol activator of LXRalpha transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; pretreating the host cell with activators of LXRalpha transcription; contacting the host cell with potential antagonists of LXRalpha transcription; and identifying compounds which block the activation the LXRalpha transcription.