发明名称 |
VERFAHREN ZUR HERSTELLUNG VON HYBRIDEM SAATGUT |
摘要 |
The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed. The invention also provides compositions and methods for high level expression of a coding region of interest in a plant. |
申请公布号 |
AT294873(T) |
申请公布日期 |
2005.05.15 |
申请号 |
AT19930810654T |
申请日期 |
1993.09.15 |
申请人 |
SYNGENTA PARTICIPATIONS AG |
发明人 |
CROSSLAND, LYLE D.;TUTTLE, ANNMARIE;STEIN, JEFFREY I. |
分类号 |
A01H1/00;A01H1/02;A01H5/00;C07K14/415;C12N5/10;C12N15/29;C12N15/82;(IPC1-7):C12N15/82 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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