发明名称 |
GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
摘要 |
The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage site, where the cleavage site intervenes between the green fluorescent protein and the first partner of the affinity couple. Further provided herein is a nucleic acid molecule which contains a nucleotide sequence encoding a tagged toxin substrate which includes (i) a fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a clostridial toxin recognition sequence containing a cleavage site, where the cleavage site intervenes between the fluorescent protein and the first partner of the affinity couple.
|
申请公布号 |
US2005100973(A1) |
申请公布日期 |
2005.05.12 |
申请号 |
US20040917844 |
申请日期 |
2004.08.13 |
申请人 |
ALLERGAN SALES, INC. |
发明人 |
STEWARD LANCE E.;GILMORE MARCELLA A.;AOKI KEI R. |
分类号 |
G01N21/78;C07K7/00;C07K7/06;C07K7/08;C07K14/00;C07K14/435;C07K14/47;C12Q1/37;G01N33/569;(IPC1-7):G01N33/554;G01N33/53;G01N33/537;G01N33/543 |
主分类号 |
G01N21/78 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|