RNA molecules have been shown to play a variety of functional roles in biological processes. RNA sequences can adopt 3-D conformations able to regulate transcription. RNA-based transcriptional regulators when recruited to a DNA template act to promote or suppress the transcription of nearby genes. The regulators are thought to act by mimicing the activation domains of protein transcriptional activators. These RNA-based transcriptional regulators may be engineered to regulate transcription based on the binding of a ligand such as a small molecule. RNA sequences of the regulators may be evolved to produce regulators with a greater degree of transcriptional activation or suppression. The RNA-based system of the present invention is useful in studying biological processes and in altering biological process in a therapeutic context.
申请公布号
WO2004113495(A3)
申请公布日期
2005.05.12
申请号
WO2004US16147
申请日期
2004.05.24
申请人
THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE;LIU, DAVID, R.;BUSKIRK, ALLEN, R.;KEHAYOVA, POLINA, D.
发明人
LIU, DAVID, R.;BUSKIRK, ALLEN, R.;KEHAYOVA, POLINA, D.