发明名称 |
RNA extraction method, RNA extraction reagent, and method for analyzing biological materials |
摘要 |
<p>A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like. <IMAGE></p> |
申请公布号 |
EP1529841(A1) |
申请公布日期 |
2005.05.11 |
申请号 |
EP20040026296 |
申请日期 |
2004.11.05 |
申请人 |
HITACHI HIGH-TECHNOLOGIES CORPORATION |
发明人 |
YAMASHITA, YOSHIHIRO;SAKURAI, TOSHINARI;KUNO, NORIHITO;UCHIDA, KENKO;YOKOBAYASHI, TOSHIAKI |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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