发明名称 |
METHODS OF SYNTHESIZING HETEROMULTIMERIC POLYPEPTIDES IN YEAST USING A HAPLOID MATING STRATEGY |
摘要 |
Methods are provided for the synthesis and secretion of recombinant hetero- multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and th en genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero- multimeric protein. |
申请公布号 |
CA2541651(A1) |
申请公布日期 |
2005.05.06 |
申请号 |
CA20042541651 |
申请日期 |
2004.10.22 |
申请人 |
KECK GRADUATE INSTITUTE;ALDER BIOPHARMACEUTICALS, INC. |
发明人 |
SCHATZMAN, RANDALL;TOLSTORUKOV, ILYA I.;LATHAM, JOHN;CREGG, JAMES M.;LITTON, MARK |
分类号 |
C12P21/04;C07K16/00;C07K16/28;C12N15/74 |
主分类号 |
C12P21/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|