发明名称 |
PARALLEL POLYMER SEQUENCING METHODS |
摘要 |
The present invention relates to a method of sequencing a target polynucleotide by enzymatic and/or chemical means. The sequencing method includes a method for characterizing multiple alleles in a sample, a method of calculating confidence levels in ascertained sequences, a method for comparing polynucleotide sequences and a method of resolving ambiguities in a polynucleotide sequence. It also provides methods for appropriately preparing samples, for immobilising template molecules, for organising the template molecules and to conduct the sequencing of many molecules in parallel. The method involves analysing molecules as members of an array. Many target polynucleotides or many segments of a single target polynucleotide can be sequenced simultaneously. In a preferred embodiment the method involves analysing individual molecules within an array and base calls are based on the signals from two or more molecules. A method to prevent non-specific signal in sequencing is also provided. The invention is readily automated, both for small-scale and large-scale operation and relevant algorithms and the composition of kits and systems are provided. |
申请公布号 |
WO2005040425(A2) |
申请公布日期 |
2005.05.06 |
申请号 |
WO2004GB04432 |
申请日期 |
2004.10.20 |
申请人 |
ISIS INNOVATION LTD;SHCHEPINOV, MIKHAIL, S.;MIR, KALIM |
发明人 |
SHCHEPINOV, MIKHAIL, S.;MIR, KALIM |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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