| 摘要 |
Systems and method for determining, based on a gray scale image, focus of an imaging system. A method of this invention includes the steps of: a) acquiring a gray scale image of a test target, b) binarizing the acquired gray scale image, c) analyzing the binarized acquired image, d) adjusting focus of acquisition optics of the imaging system, and e) repeating steps a) through d) until a substantially maximum number of line pairs of a number of groups of line pairs of the test target is obtained, wherein each group from the number of groups of line pairs has a predetermined spatial frequency.
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