发明名称 |
Method for labeled amidation of biological molecule and method for analyzing biological molecule |
摘要 |
The present invention provides a method for improving the sensitivity of mass spectrometry for biological molecules. The present invention also provides a method for a rapid and simple analysis of biological samples by making use of the method for improving the sensitivity of mass spectrometry. A method for labeled amidation, comprising the step of reacting an amidating reagent labeled with <SUP>15</SUP>N, an isotope of nitrogen, with the carboxyl group of a biological molecule. A method for analyzing a biological molecule, comprising the steps of performing amidation to a carboxyl group of a biological molecule to obtain an amidated form of the biological molecule, and subsequently subjecting the amidated form of the biological molecule to mass spectrometry. A labeled amidated form of a biological molecule wherein a carboxyl group of the biological molecule is amidated as <SUP>15</SUP>N-labeled amide group.
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申请公布号 |
US2005089951(A1) |
申请公布日期 |
2005.04.28 |
申请号 |
US20040969900 |
申请日期 |
2004.10.22 |
申请人 |
TANAKA KOICHI;SEKIYA SADANORI;WADA YOSHINAO |
发明人 |
TANAKA KOICHI;SEKIYA SADANORI;WADA YOSHINAO |
分类号 |
A61K51/00;A61K51/08;C07K1/13;C07K14/805;C12P21/06;G01N33/60;(IPC1-7):A61K51/00 |
主分类号 |
A61K51/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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