发明名称 |
NUCLEIC ACID EXTRACTION METHOD |
摘要 |
<p>The invention relates to a method for the extraction of nucleic acids from microorganisms and to the use thereof in a method for the analysis of a microbial population in a given environment, e.g. a population of microorganisms taken from the air. In particular, the invention relates to an extraction method in which the following three lysis steps are performed successively in any order, namely: (i) chemical lysis of the microorganisms, consisting in bringing the microorganisms into contact with a lysis solution containing a detergent; (ii) thermal shock lysis comprising the incubation of the extract at a temperature lower than 0 °C, immediately followed by the incubation of the extract at a temperature of at least 95 °C and preferably 100 °C; and mechanical lysis in which the extract is stirred in the presence of balls.</p> |
申请公布号 |
WO2005038025(A1) |
申请公布日期 |
2005.04.28 |
申请号 |
WO2004FR02613 |
申请日期 |
2004.10.13 |
申请人 |
BERTIN TECHNOLOGIES;MARON, PIERRE-ALAIN;LEJON, DAVID;BIZET, KARINE;CARVALHO, ESMERALDA |
发明人 |
MARON, PIERRE-ALAIN;LEJON, DAVID;BIZET, KARINE;CARVALHO, ESMERALDA |
分类号 |
C12N15/10;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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