Methods and compositions for in vitro and in vivo use of parallel stranded hairpins and triplex structures as nucleic acid ligands

摘要

The invention sets forth parallel-stranded hairpins and parallel-stranded hairpins carrying a single strand target, the parallel-stranded hairpins containing 8-aminopurine residues and are able to bind a target molecule of interest due to specificity in the two-dimensional and three-dimensional structures of the parallel-stranded hairpins and parallel-stranded hairpins carrying a single strand target. The invention also relates to creation of a library of parallel-stranded hairpins and associated triplexes for use as aptamers, as well as methods of synthesis of parallel-stranded hairpins and use of the structures of the invention to detect and eliminate molecules of interest.