CELL CULTURE SUBSTRATE AND CELL CULTURE METHOD

摘要

PROBLEM TO BE SOLVED: To provide a flexible and tough cell culture substrate hardly causing breakage of a cell and contamination of the substrate when separating and collecting the cultured cells to enable the cultured cells to be rapidly collected; and to provide a cell culture method by which the collection of the cultured cells is easily carried out.

SOLUTION: The flexible and tough cell culture substrate is achieved by a polymer of a water-soluble organic monomer, and a polymer hydrogel having a three-dimensional structure constituted of a water-swellable clay mineral. The cell culture substrate comprising the polymer hydrogel allows the cells to be suitably cultured and proliferated under a condition exhibiting hydrophobicity, and the adhesion to the cells is reduced under a condition exhibiting hydrophilicity by using the polymer hydrogel exhibiting hydrophilicity and hydrophobicity by various kinds of the setting of conditions exemplified by a temperature condition to enable the cultured cells to be easily and rapidly peeled and collected without causing the breakage of the cultured and proliferated cells, and the contamination of the peeled substrate.

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