发明名称 |
Measuring receptor homodimerization |
摘要 |
The invention provides methods and kits for detecting and/or measuring receptor homodimers on a cell surface membrane. In one aspect, the methods employ pairs of probes comprising binding compounds and a cleaving probe, such that at least one binding compound binds specifically to the same epitope of a membrane-bound analyte as the cleaving probe. The binding compound includes one or more molecular tags attached through a cleavable linkage, and the cleaving probe includes a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to a homodimer of a cell surface molecules results in release of molecular tags from the binding compounds, providing a measure of formation of the homodimeric complex.
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申请公布号 |
US2005089940(A1) |
申请公布日期 |
2005.04.28 |
申请号 |
US20040946816 |
申请日期 |
2004.09.22 |
申请人 |
PIDAPARTHI SAILAJA;SHI YINING;DUA RAJIV;CHAN-HUI PO-YING;SINGH SHARAT |
发明人 |
PIDAPARTHI SAILAJA;SHI YINING;DUA RAJIV;CHAN-HUI PO-YING;SINGH SHARAT |
分类号 |
C12Q1/68;G01N33/53;G01N33/567;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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