METHOD OF PRETREATING SAMPLE AND IMMUNOLOGICAL ASSAY METHOD USING THE SAME

摘要

It is intended to provide a method of pretreating a sample for conveniently, quickly and accurately assaying the total amount of adiponectin, which occurs as a mixture of various multimer species in a biological sample, by an immunological assay method. Namely, a method of pretreating a sample for assaying adiponectin in order to immunologically assay the total amount of adiponectin in a sample, characterized by comprising treating the sample containing adiponectin with at least one member selected from among a reducing agent, an acid or its salt, a surfactant and a protease.