METHOD FOR PREPARING OLIGONUCLEOTIDES

摘要

A method for preparing an oligonucleotide comprising the steps of a) providing a 3'-protected compound having the formula: wherein B is a heterocyclic base R2 is H, a protected 2'-hydroxyl group, F, a protected amino group, an O-alkyl group, an O-substituted alkyl, a substituted alkylamino or a C4'-O2'methylen linkage R3 is a hydroxyl protecting group, a 3'-protected nucleotide or a 3'-protected oligonucleotide b) reacting said compound with a nucleotide derivative having a 5'-proctection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond c) processing the elongated oligonucleotide with a P(III)-internucleotide bond by steps c1) and c2) in any sequence c1) capping by reacting with a solid supported capping agent c2) oxidizing by reacting the oligonucleotide with a solid supported oxidizing reagent d) removing the 5'-protection group by treatment with a solid supported agent or removing the 5'-protection group with a removal agent followed by addition of a solid supported scavenger or followed by extraction.