发明名称 |
Expression of alkaline phosphatase in yeast |
摘要 |
This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.
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申请公布号 |
US6884602(B2) |
申请公布日期 |
2005.04.26 |
申请号 |
US20010911132 |
申请日期 |
2001.07.23 |
申请人 |
ROCHE DIAGNOSTICS OPERATIONS, INC. |
发明人 |
MUELLER RAINER;THALHOFER JOHANN-PETER;GEIPEL FRANK;HOELKE WERNER;GLASER STEPHAN;ECKSTEIN HELLMUT;KIRSCHBAUM THOMAS;BOMMARIUS BETTINA |
分类号 |
C12N15/09;C12N1/19;C12N9/16;C12N15/53;C12N15/55;C12N15/81;C12R1/645;(IPC1-7):C12P21/06;C12N15/74;C12N1/14;C12N1/16;C07K1/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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