BIDERECTIONAL PROMOTERS FOR SMALL RNA EXPRESSION

摘要

The invention provides bidirectional promoters for expressing two or more short RNA sequences from a single promoter. A particular embodiment of the bidirectional promoters of the invention include: 1) a Pol III promoter that contains a TATA box, a PSE and a DSE; and 2) a Pol III promoter that includes a PSE and a TATA box fused to the 5' end of said DSE in reverse orientation. Vector embodiments are also disclosed comprising the novel bidirectional promoters of the invention, as well as methods of making and using these promoters.