发明名称 |
A METHOD FOR IN VIVO PRODUCTION OF A MUTANT LIBRARY IN CELLS |
摘要 |
<p>A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises i) an origin of replication from which replication is initiated, ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic, iii) a gene encoding the polypeptide of interest. Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.</p> |
申请公布号 |
EP0873398(B1) |
申请公布日期 |
2005.04.20 |
申请号 |
EP19970900206 |
申请日期 |
1997.01.10 |
申请人 |
NOVOZYMES A/S |
发明人 |
BORCHERT, TORBEN VEDEL;EHRLICH, STANISLAS, DUSKO |
分类号 |
C12N15/09;C12N1/15;C12N1/21;C12N5/10;C12N9/00;C12N15/10;C12R1/07;C12R1/185;C12R1/44;C12R1/46;C12R1/66;(IPC1-7):C12N1/21 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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