发明名称 |
SENSITIZER-LABELED ANALYTE DETECTION |
摘要 |
The invention provides methods for detecting an analyte in a sample including the steps of: (a) exciting a sensitizer label on an analyte; (b) permitting energy from the excited sensitizer label to be transferred to and excite an acceptor molecule, whereby the sensitizer label returns to an unexcited state; (c) reacting the excited acceptor molecule with a chemiluminescent precursor to form a chemiluminescent compound which emits light in response to an activation source; (d) exposing the chemiluminescent compound to the activating source to produce a detectable signal; (e) detecting the signal; and (f) correlating the signal with the presence or absence of the analyte. The chemiluminescent precursor is desirably an olefin capable of being converted to a 1,2-dioxetane. Target amplification techniques, such as PCR, may be used to directly label a target analyte with a sensitizer. |
申请公布号 |
EP1523668(A1) |
申请公布日期 |
2005.04.20 |
申请号 |
EP20030764344 |
申请日期 |
2003.07.03 |
申请人 |
EMP BIOTECH GMBH |
发明人 |
LEVISON, DEREK, W.K.;MOLLER, UWE;LEVISON, STUART |
分类号 |
G01N21/76;G01N33/542;(IPC1-7):G01N21/76;G01N33/533;G01N33/566 |
主分类号 |
G01N21/76 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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