METHOD OF DETECTING SINGLE GENE COPIES IN-SITU

摘要

Compositions and methods are provided for detecting single copies of a gene in-situ using brightfield microscopy in detection of various target nucleic acid sequences, such as genes or nucleic acid of microorganisms in a cell. In particular, nucleic acid probes for various target nucleic acid sequences in a cell are utilized in conjunction with enzymes and their substrates to produce chromogenic composition associated with the target nucleic acid sequences in the cell.