发明名称 |
PURIFIED ACTIVE HCV NS2/3 PROTEASE. |
摘要 |
<p>A method for producing a refolded, inactive form of recombinantly produced NS2/3 protease which comprises the steps of: a) purifying the protease from inclusion bodies in the presence of a chaotropic agent; and b) refolding the purified protease by contacting it with a reducing agent and lauryldiethylamine oxide (LDAO) in the presence of reduced concentration of chaotropic agent or polar additive. The invention further comprises a method for activating this refolded inactive NS2/3 protease by adding an activation detergent. This method produces large amounts of the active NS2/3 protease to allow small molecules and ligands to be screened as potential inhibitors of NS2/3 protease, which may be useful as therapeutic agents against HCV.</p> |
申请公布号 |
MXPA03004835(A) |
申请公布日期 |
2005.04.19 |
申请号 |
MX2003PA04835 |
申请日期 |
2001.12.13 |
申请人 |
BOEHRINGER INGELHEIM (CANADA) LTD. |
发明人 |
PAUSE, ARNIM |
分类号 |
G01N33/50;C07K14/18;C12N5/02;C12N7/01;C12N9/50;C12N15/50;C12N15/57;C12Q1/37;G01N33/15;G01N33/68;(IPC1-7):C07K1/14;G01N33/576 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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