发明名称 |
METHODS FOR PERFUSION AND PLATING OF PRIMARY HEPATOCYTES AND A MEDIUM THEREFORE |
摘要 |
The present invention provides methods for culturing primary hepatocytes with improved long term function and improved viability, by plating the hepatocytes in the presence of an anti-oxidants) as well as an agent(s) which is a functional inhibitor of enzymes that generate reactive oxygen and reactive nitrogen species. One preferred embodiment provides a combination of 2-oxo-thizolidine and tocopherol succinate. Another preferred embodiment provides a combination of NG- methylarginine and mannitol.
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申请公布号 |
KR20050035870(A) |
申请公布日期 |
2005.04.19 |
申请号 |
KR20057001490 |
申请日期 |
2005.01.27 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
LEACH, JOHN, K;TANNENBAUM, STEVEN, R. |
分类号 |
C12N5/071;G01N33/50;(IPC1-7):C12N5/00;C12N5/02;C12N5/08 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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