| 摘要 |
<PICT:1101361/C2/1> The new antibiotic, Shincomycin, active towards gram positive micro-organisms and produced by aerobic culture of Streptomyces phaeschromogenus No. 903, ATCC 19,081 or a shincomycin-producing mutant thereof in conventional media, comprises a mixture of shincomycin A and shincomycin B, each respectively having the following properties: Melting point, 134 DEG to 136 DEG C., and 128 DEG to 129 DEG C.; Analysis, C = 60.12%, H = 8.51%, O = 26.81%, N = 1.34% and C = 58.26%, H = 8.44%, O = 28.25%, N = 1.83%; Molecular weight: 1033 and 1062; Molecular formula: C52H89O19N and C52H90O20N; Specific rotation, [a ]D, C. 1.25 in chloroform = - 61.2 DEG and/sB0 DEG ; Infra-red spectra, as in Fig.1 and Fig. 2; Solubility, both are readily soluble in benzene, acetone, ethanol, methanol, and ethyl acetate, slightly soluble in CS2, and ether, and insoluble in water and petroleum ether; they both form colourless crystals when pure, and have an ultra-violet absorption maximum at 240 mm . Suitable culture conditions are by surface, shaken, or submerged aerated culture, at a pH of 5 to 8, and a temperature of 20 DEG to 35 DEG C. The antibiotic mixture may be isolated from the filtered culture broth by making the latter alkaline or slightly acid, e.g. above pH 6.0, and extracting with a water-immiscible organic solvent. Alternatively, the shincomycin may be adsorbed on, e.g. active charcoal or clay, and extracted therefrom with a water-immiscible or slightly miscible organic solvent or an aqueous acid solution. The antibiotic may also be precipitated with suitable acids, e.g. oxalic, tartaric or picric, as an insoluble salt. Further purification comprises chromatography on alumina or silica gel and elution with benzene/chloroform or chloroform/methanol. The antibiotic may be separated into components A and B by thin layer chromatography or counter current phophate buffer/insoluble organic solvent distribution, and finally purified by crystallization.
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