发明名称 |
FOREIGN PAS LIGANDS REGULATE PAS DOMAIN FUNCTION |
摘要 |
Specific binding of a foreign core ligand to a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is determined by (a) detecting a first NMR spectrum of the PAS domain in the presence of a foreign ligand; and (b) comparing the first NMR spectrum with a second NMR spectrum of the PAS domain in the absence of the ligand to infer the presence the ligand specifically bound within the hydrophobic core of the PAS domain. A functional surface binding specificity of a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain. |
申请公布号 |
WO2005033662(A2) |
申请公布日期 |
2005.04.14 |
申请号 |
WO2004US32417 |
申请日期 |
2004.10.01 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
GARDNER, KEVIN;ERBEL, PAUL;CARD, PAUL;RUTTER, JARED;HARPER, SHANNON;AMEZCUA, CARLOS;BRUICK, RICHARD;MCKNIGHT, STEVE |
分类号 |
C07H21/04;C12N15/29 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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