TRANSFORMED CELL, PROCESS FOR PRODUCING PROTEIN USING THE CELL, PROTEIN PRODUCTION KIT, DNA FRAGMENT FOR EXPRESSING VIRUS VECTOR, USE OFTHE SAME, PROCESS FOR PRODUCING TRANSFORMANT FOR PROTEIN PRODUCTION, TRANSFORMANT FOR PROTEIN PRODUCTION OBTAINED BY THE METHOD AND UTILIZATION THEREOF

摘要

An expression vector is constructed by transferring recombinant tomato mosai c virus (ToMV) cDNA, in which a coat protein of ToMV having a suppressor for a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring t he above expression vector into a tobacco BY-2 cell, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.