发明名称 METHODS OF ESTABLISHING INTERCELLULAR COMMUNICATION IN AN ENDOTHELIAL CELL LAYER AND USES THEREOF
摘要 The present invention provides methods for generating endothelial cells in vitro, with functional intercellular communication (e.g. GJIC) that is consistent with normal endothelial cells and cell layers in vivo. In accordance with the invention, expression and appropriate organization of gap junctional proteins in endothelial cells is achieved through a) biophysical engineering, b) genetic engineering, or c) a combination of both biophysical engineering and genetic engineering. The fully functioning endothelial cells generated in vitro, in accordance with the invention are suitable for use in conjunction with substrates and matrices commonly used in tissue engineering of vascular implants and are of the prior art.
申请公布号 WO2005033292(A2) 申请公布日期 2005.04.14
申请号 WO2004US32577 申请日期 2004.10.04
申请人 RENSSELAER POLYTECHNIC INSTITUTE;THE CHILDREN'S HOSPITAL OF PHILADELPHIA;DEPAOLA, NATACHA;POLACEK, DENISE 发明人 DEPAOLA, NATACHA;POLACEK, DENISE
分类号 A61K35/12;C12N;C12N5/00;C12N5/071 主分类号 A61K35/12
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