METHODS OF RAPID DETECTION AND IDENTIFICATION OF BIOAGENTS USING microRNA
摘要
Methods for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of microRNA containing nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of microRNA containing nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a "base composition signature" (BCS) or molecular mass which is then matched against a database of base composition signatures or molecular masses, by which the species of the bioagent is identified.
申请公布号
WO2005033271(A2)
申请公布日期
2005.04.14
申请号
WO2004US28869
申请日期
2004.09.07
申请人
ISIS PHARMACEUTICALS, INC.;GRIFFEY, RICHARD, H.;ECKER, DAVID, J.