发明名称 |
Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids |
摘要 |
Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
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申请公布号 |
US2005079618(A1) |
申请公布日期 |
2005.04.14 |
申请号 |
US20040841125 |
申请日期 |
2004.05.07 |
申请人 |
THE GOVERNMENT OF THE USA AS REPRESENTED BY THE SECRETARY OF THE DEPT OF HEALTH AND HUMAN SERVICES |
发明人 |
COURT DONALD L.;LI XIN-TIAN;HUANG JIAN-DONG;COSTANTINO NINA;LIU DEPEI |
分类号 |
C12N1/20;C12N15/70;(IPC1-7):C12N15/74 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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