发明名称 |
Pharmacologically active novel dauer pheromone compound for controlling aging and stress and method for isolating and characterizing the same |
摘要 |
The present invention discloses a method for effectively isolating and purifying a physiological active dauer pheromone compound having the following structural formula I related to aging and stress of C. elegans, and determination of the structure of such material. First, ethanol extract is prepared by completely concentrating a liquid medium with ethanol after culturing C. elegans on a S. basal liquid medium, and then this ethanol fraction is further extracted with ethyl acetate. Then, impurities are removed from ethyl acetate extract by a silica-gel adsorption chromatography and a pheromone extract is isolated and purified by amine column chromatographic procedures using various solvents as eluent. Finally, a pheromone compound having structural formula I is completely isolated and characterized by size-exclusion HPLC.
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申请公布号 |
US2005075389(A1) |
申请公布日期 |
2005.04.07 |
申请号 |
US20040490837 |
申请日期 |
2004.03.25 |
申请人 |
PAIK YOUNG KI;JEONG PAN YOUNG |
发明人 |
PAIK YOUNG KI;JEONG PAN YOUNG |
分类号 |
C07D309/12;C07D309/10;C07H15/04;(IPC1-7):A01N43/16;C07D39/10 |
主分类号 |
C07D309/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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