| 摘要 |
Non-fluorescent dyes (i.e., dark quenchers) which can be used to quench the fluorescence of energy donors in bioassays through fluorescence resonance energy transfer (FRET) are described. The dark quenchers can be associated with (e.g., conjugated to) peptides, proteins, antibodies, DNA/RNA, or other biological molecules or receptors or complexed to metal containing compounds to develop bioassays based on donor-acceptor energy transfer. Bioassays are also described wherein an increase or a decrease in separation distance between a fluorescent donor compound and a dark quencher or dark quencher conjugate is detected. Kits including the dark quenchers or dark quencher conjugates are also described. |
