METHODS OF SYNTHESIZING POLYNUCLEOTIDES USING THERMOSTABLE ENZYMES

摘要

Disclosed are methods of synthesizing a polynucleotide complementary to a target polynucleotide include steps of subjecting a non­-thermophilic cell comprising a thermostable polymerase to a temperature effective to disrupt the cell to form a reaction mixture, wherein the reaction mixture comprises the target polynucleotide and one or more primers that hybridize to a sequence of the target polynucleotide or to a sequence flanking the polynucleotide and incubating the reaction mixture under conditions whereby the polynucleotide is synthesized. Also disclosed are cell libraries and kits in accordance with the invention.