STANDARDIZED AND OPTIMIZED REAL-TIME QUANTITATIVE REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION METHOD FOR DETECTION OF MRD IN LEUKEMIA
摘要
The invention relates to in a real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) method for minimal residual disease detection in leukemic patients through amplification of a fusion gene transcript, comprising: (i) selecting amplifiable and qualified patient samples for subsequent analysis; (ii) defining optimal conditions for performing the RT reaction; (iii) defining optimal conditions for RQ-PCR protocol; and (iv) establishing a standardized procedure for data analysis.
申请公布号
WO2004078020(A3)
申请公布日期
2005.03.31
申请号
WO2004EP04008
申请日期
2004.03.08
申请人
UNIVERSITE DE LA MEDITERRANEE;GABERT, JEAN;BEILLARD, EMMANUEL;BI, WANLI;VAN DONGEN, JACQUES
发明人
GABERT, JEAN;BEILLARD, EMMANUEL;BI, WANLI;VAN DONGEN, JACQUES