METHOD FOR FUNCTIONALLY SCREENING AGENTS WHICH CAN MODULATE THE ACTIVITY OF UBIQUITINE HYDROLASES AND USES THEREOF.

摘要

A cell-screening method for compounds which can modulate the activity of ubiquitine hydrolases comprising the following stages: cultivation of a strain of modified yeast in a rich medium, comprising a hybrid sequence containing a sequance coding for a protein (Met4) and a transcriptional reporter system containing a a yeast promoter activated by the transcriptional factor (Met4), the transfer of the culture into a rich medium over a period of time enabling expression of the protein (Met4), transfer of the culture into a medium which does not enable expression of the protein (Met4), bringing the strain of yeast thus cultivated into contact with a test compound, the addition of cadmium, and determination of the level of expression of the hybrid protein expressed by a promoter MET.