发明名称 |
High throughput screening for novel enzymes |
摘要 |
Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nuclei acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
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申请公布号 |
US2005069917(A1) |
申请公布日期 |
2005.03.31 |
申请号 |
US20040842187 |
申请日期 |
2004.05.10 |
申请人 |
SHORT JAY M.;KELLER MARTIN |
发明人 |
SHORT JAY M.;KELLER MARTIN |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;C40B40/02;(IPC1-7):C12Q1/68;G01N33/53 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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