Methods of screening, determining, developing and utilizing negative cross resistance toxins

摘要

A method is provided for evaluating the efficacy of a molecule against a target population, the target population, including a strain resistant to a first toxin. The method comprising determining a susceptible strain in the target population. The susceptible strain being susceptible to the first toxin. The method further comprising selecting for a resistant strain in the target population. The resistant strain being resistant to the first toxin. The method further comprising evaluating the efficacy of the resistant strain with a plurality of molecules to determine a second toxin that is more toxic to the resistant strain than to the susceptible strain. Wherein the target population is at least one of an insect, a fungi, a plant, and a nematode. Methods and systems are provided for determining and developing negative cross resistance factors. The method includes evolving a strengthened NCR toxin from an initial NCR toxin by obtaining an initial NCR compound, selectively increasing the toxicity of the NCR compound, and testing the evolved compound to determine if the evolved compound is a stronger NCR compound than the initial NCR toxin. Methods and systems are also provided for deployment and commercial use of NCR toxins.