| 摘要 |
FIELD: molecular biology, biotechnology, biochemistry, pharmaceutical chemistry. ^ SUBSTANCE: invention relates to new unnatural substrates for enzyme terminal deoxynucleotidyl transferase that can be used as a tool for insertion of label by 3'-hydroxyl group of oligodeoxynucleotides and single-stranded DNA. Invention describes new substrates for terminal deoxynucleotidyl transferase of the general formula (I): wherein R represents a marker group chosen from dansyl, dinitrophenyl, fluorescein, fluorenylmethyloxycarbonyl, rhodamine B, tetramethylrhodamine, eosin, erythrosine, trioxalene, BODIPY, biotin, auramine, psoralene, pyrene, Texas red dye, actinomycin D, 7-methoxycoumarin and ellipticin; X is a linker representing a linear carbon chain that comprises heteroatoms, such as nitrogen and oxygen atoms, and this chain can comprise carbonyl and thiocarbonyl as functional groups. Invention provides preparing new effective substrate for enzyme. ^ EFFECT: valuable biochemical properties of substrates. ^ 1 tbl, 2 dwg, 7 ex |
