METHOD FOR ASSAY OF SOFT WHEAT FLOUR IMPURITY IN GRIT (SEMOLINE) OF DURUM WHEAT AND IN READY PRODUCTION OF MACARONI-MAKING INDUSTRY

摘要

FIELD: food industry. ^ SUBSTANCE: for assay of soft wheat flour impurity in grit (semolina) of durum wheat polymerase chain reaction (PCR) is carried out for the presence of DNA sequence specific for genome of soft wheat (T. destivum). PCR is carried out using pair of oligonucleotide primers with homology to region of external transcribing spacer of highly repeating genes encoding ribosomal RNA in locus NorD3 belonging to genome D of soft wheat only. In the case the presence of corresponding DNA sequence of size 791 nulceotide pairs in products of PCR reaction the conclusion can be made about the presence of impurity ("adulteration") of grit with durum wheat flour (T. durum) with soft wheat. Invention can be used for assay of quality of macaroni articles and raw for their preparing. ^ EFFECT: improved assay method. ^ 3 dwg