发明名称 |
DNA DETECTION METHOD USING MOLECULAR BEACON WITH THE USE OF MONOMER FLUORESCENCE/EXCIMER FLUORESCENCE SWITCHING OF FLUORESCENT MOLECULE |
摘要 |
<p>It is intended to provide a means and a method whereby a molecule for DNA detection at an elevated accuracy can be provided, different from the existing DNA detection system with the use of a molecular beacon. A molecular beacon wherein fluorescent organic groups capable of forming an excimer are bonded to the 3'- and 5'-ends of a single-stranded oligonucleotide to be hybridizable with a subject oligonucleotide and the switching of monomer emission/excimer emission is utilized; and a method of detecting a complementary strand DNA sequence and a method of detecting SNP by using this molecular beacon.</p> |
申请公布号 |
WO2005026390(A1) |
申请公布日期 |
2005.03.24 |
申请号 |
WO2004JP11819 |
申请日期 |
2004.08.18 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY;INOUYE, MASAHIKO;FUJIMOTO, KAZUHISA;SHIMIZU, HISAO |
发明人 |
INOUYE, MASAHIKO;FUJIMOTO, KAZUHISA;SHIMIZU, HISAO |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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