| 摘要 |
A method for preserving sperm comprising: (a) cooling a first sample solution which includes a sperm sample to a first temperature sufficient to protect sperm from glycerol toxicity, at a rate sufficiently slow that the metabolic rate of sperm is decreased, to provide a cooled first sample solution, maintained for a period of time at the first temperature; (b) adding a solution comprising glycerol to the cooled first sample solution; (c) mixing the glycerol and the cooled first sample solution together to comprise a second sample solution; (d) lowering the temperature of the second sample solution to a second temperature for a sufficient period of time to equilibrate glycerol and sperm; and (e) freezing the second sample solution to thereby provide a frozen sperm sample, such that the sperm is preserved; wherein the first sample solution does not contain glycerol; wherein the second sample solution further comprises an antibiotic compound, such as tylosin, gentamicin, lincospectin or spectinomycin; wherein the sperm sample is cooled to the first temperature and maintained at the first temperature for 4 to 12 hours; wherein the second sample solution is allowed to freeze at the second temperature.
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